MYLK Antibody

• Catalog Number: orb1262835
• Category: Antibodies
• Description: MYLK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 908-938 amino acids from the N-terminal region of human MLCK.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 211 kDa
• Target: MYLK
• UniProt ID: Q15746
• NCBI: Q15746
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Myosin light chain kinase, smooth muscle, MLCK, sm
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1) at 1:1000.

Western blot analysis in SK-BR-3 cell line lysates (35 ug/lane).This demonstratesdetected the MLCK protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long was detected using purified Pab.