MYF5 Peptide

• Catalog Number: orb1233851
• Category: Proteins
• Description: MYF5 Peptide
• Tested applications: Blocking
• Form/Appearance: Lyophilized
• Target: MYF5
• UniProt ID: P13349
• NCBI: NP_005584.1
• Storage: Store at -20°C. When reconstituted minimize freezing and thawing.
• Buffer/Preservatives: MYF5 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
• Alternative names: bHLHc2, BHLHC2, Myogenic factor 5, Class C basic h
• Note: For research use only
• Application notes: MYF5 Peptide is used for blocking the activity of MYF5 antibody (catalog number 45-926). The MYF5 antibody ships at 0.5 mg/mL and the MYF5 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
• Expiration Date: 6 months from date of receipt.

Western blot analysis of MYF5 in Human Skeletal Muscle Lysate (35 μg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using MYF5 antibody (0.5 μg/mL).