Myeloperoxidase/MPO Antibody

• Catalog Number: orb402306
• Category: Antibodies
• Description: Myeloperoxidase/MPO Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MPO (714-728aa KNNIFMSNSYPRDFV), different from the related mouse and rat sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 83869 MW
• UniProt ID: P05164
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kD
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of MPO using anti-MPO antibody.Lane 1:human HL-60 cell; 2:rat thymus tissue; 3:mouse spleen tissue; 4:mouse thymus tissue.

IF analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of human spleen tissue.

IF analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of human appendicitis tissue.

IHC analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of human tonsil tissue.

IHC analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of mouse spleen tissue.

IHC analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of rat lymphaden tissue.