Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Myeloid Cell antibody for each application should be determined by the researcher.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Key Properties
−
Antibody Type
Primary Antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone No.
SPM298
Immunogen
Human PBMs were used as the immunogen for this Myeloid Cell antibody.
Purification
Protein G affinity chromatography
Conjugation
Unconjugated
Storage & Handling
−
Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance
Liquid
Buffer/Preservatives
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.