Myc Tag Antibody

• Catalog Number: orb1530834
• Category: Antibodies
• Description: Myc Tag Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ELISA, ICC, IP, WB
• Immunogen: residues 410-419 (EQKLISEEDL) of human myc
• Concentration: 250 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:50,000; for coating plates 1:100 - 1:500
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Myc Tag
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of Myc-tagged protein by WB from non-transfected human HEK293 cells, HEK293 transfected with Met-Myc-IDO1, HEK293 with IDO1-Myc (C-terminal Tag), and HEK293 with IDO1-Myc-HIS (Internal Tag), using antibody at 1:1000.

Detection of Myc Tag by western blot of immunoprecipitates from HEK293 transfected with Met-Myc-IDO1 lysate.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged nuclear protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.