MX1 Antibody / MxA / Myxovirus resistance protein 1

• Catalog Number: orb1824071
• Category: Antibodies
• Description: The Dynamin family of microtubule-associated proteins function as GTPases that are involved in microtubule bundling and endocytosis. In mice, Mx2 (myxovirus resistance protein 2) and Mx1 (myxovirus resistance protein 1) are members of the Dynamin family that are involved in the immune response to viral infections. Localized to the cytoplasm, Mx2 contains one GED domain and is expressed in response to viral infection or treatment by IFN-a/IFN-b. Once expression is induced, Mx2 accumulates in the cytoplasm and inhibits the replication of vesicular stomatitis virus (VSV), thereby conferring resistance to VSV infection. Unlike Mx2, Mx1 is localized to the nucleus where, upon induction by IFN-a/IFN-b, it provides selective resistance to infection by the highly lethal H5N1 influenza virus. In humans, MxA and MxB function in a similar manner to Mx1 and Mx2, conferring resistance to specific target viruses. Mx3 is a rat-specific member of the myxovirus resistance protein family.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 400-592) from the human protein was used as the immunogen for the MX1 antibody.
• UniProt ID: P20591
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: MX1/7527
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This MX1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human ovarian cancer tissue with MX1 antibody (clone MX1/7527). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with MX1 antibody (clone MX1/7527). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MX1 antibody (clone MX1/7527) as confirmation of integrity and purity.