Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

SKU: orb2302692

Description

This Multi-rAb antibody targets Mouse IgG (H+L) in FC and IF. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

Images & Validation

• Tested Applications: FC, IF
• Dilution range: 1:200-1:1000 for IF and FC
• Reactivity: Mouse

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Goat
• Clonality: Recombinant
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.

Storage & Handling

• Storage: Store at -20°C. Stable for one year after shipment.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (1:1000, orange). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302686, 1:500) and Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302692, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (1:200, green) and mouse anti-NPM1 monoclonal antibody (1:1000, orange). Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302686, 1:500) and Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302692, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (1:200, orange). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (orb2302686, 1:500) and Multi-rAb CoraLite Plus 555 conjugated Recombinant Goat anti-mouse secondary antibody were used for detection (orb2302692, 1:500).

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (1:200, green) and mouse anti-NeuN monoclonal antibody (orange). Multi-rAb CoraLite Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (orb2302686, 1:500) and Multi-rAb CoraLite Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (orb2302692, 1:500).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody ( Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302692).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (Clone: UCHT4) and Mouse IgG2a Isotype Control. Multi-rAb CoraLite Plus 550-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302692 was used at 1:500 for detection.