Multi-Cytokeratin Antibody [SPM583]

SKU: orb1251856

Description

Multi-Cytokeratin Antibody [SPM583]

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: SPM583
• Immunogen: A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.
• Target: KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18
• Purification: Protein G affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human colon carcinoma stained with anti-Multi Cytokeratin antibody (SPM583).

Quick Database Links

Gene Symbol

KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18

UniProt

P13645, P19013, P13647, P05787, P04259, P02538, P05783, P48668, P13646