MUC5AC Antibody

SKU: orb388468

Description

Mouse monoclonal antibody to MUC5AC, this monoclonal antibody (MAb) recognizes the peptide core of gastric mucin M1 (identified as MUC5AC, ~1,000 kDa). The epitope is sensitive to β‑mercaptoethanol and protease digestion, but resistant to periodate treatment. The antibody reacts with normal gastric epithelium throughout the human gastrointestinal tract, as well as with precancerous and cancerous colon tissues; however, it does not stain normal adult colonic mucosa. Positive reactivity is also observed in fetal colonic mucosa. The reappearance of gastric mucin immunoreactivity during colorectal carcinogenesis corresponds to the re‑expression of the peptide epitope characteristic of gastric (or fetal colonic) mucins.

Research Area

Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution Range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, IHC-P: 1-2 μg/ml
• Reactivity: Feline, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  MUC5AC (Mucin 5AC / Gastric Mucin)

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 / Kappa
• Clone No.: SPM297
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Target: MUC5AC
• Source/Expression System: HEK293 cells
• Molecular Weight: 1,000 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Immunohistochemical staining of human Gastric Carcinoma tissue using MUC5AC antibody

Quick Database Links

Gene Symbol

MUC5AC

UniProt

P98088