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Catalog Number | orb1788072 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | 878-911 aa |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, FC: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 99351 Da |
Target | This MTTP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 878-911 amino acids from the C-terminal region of human MTTP. |
UniProt ID | P55157 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Microsomal triglyceride transfer protein large sub Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of Hela cells using MTTP Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
All lanes: Anti-MTTP Antibody (C-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: human liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 99 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.