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Catalog Number | orb2292568 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant MTM1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1C10 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a Kappa |
Immunogen | MTM1 (AAH30779, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGAT |
NCBI | AAH30779 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged MTM1 is approximately 1 ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western Blot analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10. Lane 1: MTM1 transfected lysate (69.9 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody (M01), clone 1C10 (Cat # orb2292568). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (36.63 KDa).