MTHFR Antibody

• Catalog Number: orb1524488
• Category: Antibodies
• Description: MTHFR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MTHFR
• UniProt ID: P42898
• NCBI: NP_005948.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 5,10-methylenetetrahydrofolate reductase (NADPH);
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MTHFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFR antibody orb1524488 used for IP at 3 µg per reaction.

Detection of human MTHFR by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MTHFR (Cat. No. orb1524488) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB.