MTAP Antibody

• Catalog Number: orb2642233
• Category: Antibodies
• Description: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: SMTP-1R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant MTAP antibody is available for research use only.
• UniProt ID: Q13126
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant MTAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HeLa cell lysate with recombinant MTAP antibody (clone SMTP-1R). Expected molecular weight: 26-38 kDa (multiple isoforms).

IHC staining of FFPE human kidney with recombinant MTAP antibody (clone SMTP-1R). Required HIER: steam section in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool prior to staining.