MSK1/RPS6KA5 Antibody

• Catalog Number: orb234314
• Category: Antibodies
• Description: MSK1/RPS6KA5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 89865 MW
• UniProt ID: O75582
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ribosomal protein S6 kinase alpha-5;S6K-alpha-5;2.
• Note: For research use only
• Application notes: WB: The detection limit for MSK1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of MSK1 using anti-MSK1 antibody.Lane 1:human HEL cell; 2:rat brain tissue; 3:mouse brain tissue.

IF analysis of MSK1 using anti-MSK1 antibody. MSK1 was detected in an immunocytochemical section of T47D cells.

IHC analysis of MSK1 using anti-MSK1 antibody. MSK1 was detected in a paraffin-embedded section of Mouse Cardiac Muscle tissue.

IHC analysis of MSK1 using anti-MSK1 antibody. MSK1 was detected in a paraffin-embedded section of Rat Brain tissue.

IHC analysis of MSK1 using anti-MSK1 antibody. MSK1 was detected in a paraffin-embedded section of Human Placenta tissue.