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Catalog Number | orb1248128 |
---|---|
Category | Antibodies |
Description | MSI2/musashi-2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-SPDSLRDYFSK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3ug/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 37kDa |
Target | MSI2 / musashi-2 |
UniProt ID | Q96DH6 |
NCBI | NP_620412.1, NP_733839.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | MSI2, musashi RNA-binding protein 2, MSI2H, RNA-bi Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3ug/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248128 (0.1 ug/ml) staining of Kelly lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248128 (0.3 ug/ml) staining of fetal Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248128 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Canine | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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