MRP8/S100A8 Antibody

• Catalog Number: orb316605
• Category: Antibodies
• Description: MRP8/S100A8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, MouseImmunocytochemistry/Immunofluorescence, 2μg/ml, Mouse Flow Cytometry, 1-3μg/1x106 cells, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 10295 MW
• UniProt ID: P27005
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein S100-A8;Calgranulin-A;Chemotactic cytokine
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of S100A8 using anti-S100A8 antibody.

Flow Cytometry analysis of HEPA 1-6 cells using anti-S100A8 antibody.

IF analysis of S100A8 using anti-S100A8 antibody.

IHC analysis of S100A8 using anti-S100A8 antibody.

IHC analysis of S100A8 using anti-S100A8 antibody.