MRGPRX4 antibody

• Catalog Number: orb515458
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MRGPRX4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MRGX4. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MRGPRX4
• Entrez: 117196
• UniProt ID: Q96LA9
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: MRGX4; SNSR5; SNSR6; Mas-related G-protein coupled
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MRGX4 expression in U87MG (A), C6 (B), MCF7 (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)

Immunofluorescent analysis of MRGX4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.