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Catalog Number | orb763363 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to mPlum |
Clonality | Monoclonal |
Clone Number | PAb (919) |
Tested applications | FC, ICC, WB |
Isotype | Rabbit polyclonal |
Immunogen | mPlum from Discosoma sp. |
Concentration | 1 mg/ml |
Dilution range | FC: 1-4 μg/ml, WB: 1-2 μg/ml |
Purity | Purified by ligand affinity chromatography. |
Conjugation | Unconjugated |
Target | mPlum |
UniProt ID | Q5S3G7 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular staining or intracellular staining - depending on expression. Western blotting: Recommended dilution: 1-2 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained using anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).
Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.
Western blotting analysis of mPlum fluorescent protein using rabbit polyclonal antibody PAb (919) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.
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