Mouse VEGF R1 / Flt-1 Protein

• Catalog Number: orb420098
• Category: Proteins
• Description: Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.
• Reactivity: Mouse
• Tag: C-mFc(mIgG2a)
• Form/Appearance: Powder
• Purity: 85%
• Conjugation: Unconjugated
• MW: 109.8 kDa
• Target: VEGF R1
• Source: Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (orb420098) is expressed from human 293 cells (HEK293). It contains AA Tyr 23 - Glu 759 (Accession # P35969-1).
• Expression System: HEK293
• Biological Activity: Loaded Biotinylated Mouse VEGF164, His, on SA Biosensor, can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Tyr 23 - Glu 759
• Endotoxins: 0.1 EU per μg
• NCBI: NP_034358.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, pH7.5
• Alternative names: FLT,VEGFR1,FLT1
• Note: For research use only
• Application notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 109.8 kDa. The protein migrates as 140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Biotinylated Mouse VEGF164, His, Avitag on SA Biosensor, can bind Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. orb420098) with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).