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Item 1 of 4
Item 1 of 4
Mouse Raf1 Antibody (N-term)
Catalog Number: orb1935959
| Catalog Number | orb1935959 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34873 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 72917 Da |
| Target | This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1. |
| UniProt ID | Q99N57 |
| NCBI | NP_084056.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | RAF proto-oncogene serine/threonine-protein kinase Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Mouse Raf1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).
Mouse Raf1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).
Mouse Raf1 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Mouse Raf1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Mouse Raf1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
