Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit

• Catalog Number: orb1950076
• Category: Assays and Kits
• Description: Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit
• Reactivity: Mouse
• Range: 78.13-5000 pg/mL
• UniProt ID: E2JF22
• Sensitivity: 21.3 pg/mL
• Alternative names: PIEZO1; FAM38A; KIAA0233
• Note: For research use only
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.