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    Mouse OXB(Orexin B) ELISA Kit

    Catalog Number: orb1946782

    DispatchUsually dispatched within 5-10 working days
    $ 335.00
    Catalog Numberorb1946782
    CategoryAssays and Kits
    DescriptionMouse OXB(Orexin B) ELISA Kit
    ReactivityMouse
    Range0.16-10ng/mL
    TargetOXB
    UniProt IDO55241
    Sensitivity0.1 ng/mL
    Alternative namesHypocretin-2
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    NoteFor research use only
    Application notesThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse OXB. During the reaction, Mouse OXB in the sample or standard competes with a fixed amount of Mouse OXB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse OXB. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse OXB in tested samples can be calculated by comparing the OD of the samples to the standard curve.
    Sample Typesserum, plasma, Tissue homogenate and Other biological samples
    Expiration DatePlease enquire.
    Mouse OXB(Orexin B) ELISA Kit

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