You have no items in your shopping cart.
Cart summary
| Catalog Number | orb775494 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neuromedin B(NMB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuromedin B(NMB). |
| Tested applications | ELISA |
| Reactivity | Mouse |
| Range | 15.63-1000 pg/mL |
| Assay Time | 2.5h |
| Concentration | 1000 pg/mL |
| UniProt ID | Q9CR53 |
| Sensitivity | 4.87 pg/mL |
| Alternative names | NM-B ELISA Kit Read more... |
| Note | For research use only |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NMB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NMB in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |
