Mouse MIP2 protein

• Catalog Number: orb80132
• Category: Proteins
• Description: GRO-Beta Mouse Recombinant also caled mouse MIP-2 produced in E.coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7849 Dalton. The CXCL2 is purified by proprietary chromatographic techniques.
• Target: mGRO b
• Conjugation: Unconjugated
• Buffer/Preservatives: The protein was lyophilized with no additives.
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Val-Ala-Ser.
• Tested applications: FA, HPLC, SDS-PAGE
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of GRO-b as Reference Standard.
• Biological Origin: E.coli
• Biological Activity: The Biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with > 5 ng/ml of recombinant mouse MIP-2.
• Solubility (25°C): It is recommended to reconstitute the lyophilized GRO-beta Mouse in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Macrophage inflammatory protein 2-alpha protein, M
• Note: For research use only
• Expiration Date: 6 months from date of receipt.