Mouse Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit

SKU: orb777584

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule(MCAM).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MCAM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.242 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD146 ELISA Kit, MUC18 ELISA Kit, Cell surface glycoprotein P1H12 ELISA Kit, Melanoma-associated antigen A32 ELISA Kit, S-endo 1 endothelial-associated antigen ELISA Kit

Quick Database Links

UniProt

Q8R2Y2

References

Ahmad Joshkon 1, Wael Traboulsi 2, Magali Terme 3, Richard Bachelier 4, Hussein Fayyad-Kazan 5, Françoise Dignat-George 6, Alexandrine Foucault-Bertaud 7, Aurelie S Leroyer 6, Nathalie Bardin 8, Marcel Blot-Chabaud 7 Soluble CD146 cooperates with VEGF-A to generate an immunosuppressive microenvironment in CD146-positive tumors: interest of a combined antibody-based therapy Mol Cancer Ther, (2024)

PubMed: 39431288

Applications

ELISA

Reactivity

Mouse