Mouse Macrophage Colony Stimulating Factor 1 (MCSF) Microsample Fast ELISA Kit

SKU: orb1806651

Description

Mouse Macrophage Colony Stimulating Factor 1 (MCSF) Microsample Fast ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCSF. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse MCSF. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse MCSF. You can calculate the concentration of Mouse MCSF in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples；Sample volume：25μL
• Assay Type: Sandwich
• Assay Time: 3H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

M-CSF, CSF1, CSF-1

Quick Database Links

Gene Symbol

MCSF

UniProt

P07141