Mouse Insulin (INS) ELISA Kit

SKU: orb1807889

Description

Mouse Insulin (INS) ELISA Kit

Research Area

Metabolism Research

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 3.13-200pg/ml
• Sensitivity: 1.88 pg/ml

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IDDM2;ILPR;IRDN;MODY1

Standard curve for orb1807889 ELISA kit.

Quick Database Links

Gene Symbol

INS

UniProt

P01325, P01326

Dina M. Abo-Elmatty, Eman A. Ahmed, Mona K. Tawfik, Seham A. Helmy Metformin enhancing the antitumor efficacy of carboplatin against Ehrlich solid carcinoma grown in diabetic mice: Effect on IGF-1 and tumoral expression of IGF-1 receptors International Immunopharmacology, 44, 72 (2017)

PubMed: 28088698

Applications

ELISA

Reactivity

Mouse