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Catalog Number | orb80071 |
---|---|
Category | Proteins |
Description | IL11 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19.2kDa. The Mouse IL-11 is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | mIL 11 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 11 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse 7TD1 was found to be less then 1.5 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution with no additives. |
Alternative names | AGIF protein, Adipogenesis inhibitory factor prote Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL11 protein
> 97% as determined by SDS-PAGE and HPLC. | |
19.1 kDa | |
E.Coli |
Unconjugated | |
90% | |
45.6 kDa | |
Mouse IL-11 Protein, Fc Tag (orb1496246) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P47873). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |