Mouse IL-2 R alpha / CD25 Protein

• Catalog Number: orb570281
• Category: Proteins
• Description: Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
• Reactivity: Mouse
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 26.6 kDa
• Target: IL-2 R alpha
• Source: Mouse IL-2 R alpha, His Tag (orb570281) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Lys 236 (Accession # P01590-1).
• Expression System: HEK293
• Biological Activity: Loaded Mouse IL-2 R alpha, His Tag on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 21.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Glu 22 - Lys 236
• Endotoxins: 1.0 EU per μg
• NCBI: NP_032393.3
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL2RA,CD25,p55,IL2-RA,IL-2-RA
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Mouse IL-2 R alpha, His tag (Cat. No. orb570281) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 21.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).