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Mouse IgM Lambda isotype Control Antibody
Catalog Number: orb343769
| Catalog Number | orb343769 |
|---|---|
| Category | Proteins |
| Description | Mouse IgM Lambda isotype Control Antibody |
| Isotype | IgM |
| Conjugation | Unconjugated |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE. |
| Tested applications | FC, SDS-PAGE |
| Dilution range | FC: 1:1000-1:5000 |
| Application notes | Mouse IgM lambda isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
| Source | Mouse |
| Biological Origin | Mouse |
| Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Alternative names | Mouse IgM Lambda isotype Control, Mouse IgM Lambda Read more... |
| Note | For research use only |
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE of Mouse IgM lambda isotype control. Lane 1: 1x Reduced Mouse IgM lambda. Lane 2: Opal Prestained Standard. Lane 3: 1X Non-reduced Mouse IgM lambda. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.
