Mouse IgG3 Kappa isotype Control Antibody

• Catalog Number: orb343767
• Category: Proteins
• Description: Mouse IgG3 Kappa isotype Control Antibody
• Isotype: IgG3
• Conjugation: Unconjugated
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 0.953 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Mouse IgG3 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
• Tested applications: FC, SDS-PAGE
• Dilution range: FC: 1:1000-1:5000
• Application notes: Mouse IgG3 kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Mouse IgG3 isotype control, Mouse IgG3 subclass is
• Note: For research use only

SDS-PAGE results of Mouse IgG3 kappa Isotype Control. Lane 1: MOUSE IgG3 Kappa (K) isotype control [1.0 µg] Reduced. Lane 2: MOUSE IgG3 Kappa (K) isotype control [1.0 µg] Reduced. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: MOUSE IgG3 Kappa (K) isotype control [1.0 µg] Non-Reduced. Lane 5: MOUSE IgG3 Kappa (K) isotype control [1.0 µg] Non-Reduced. 4-20% Gel, Coomassie Stained.