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Catalog Number | orb347576 |
---|---|
Category | Antibodies |
Description | Mouse IgG2b (Gamma 2b chain) antibody (Alkaline Phosphatase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Mouse IgG2b heavy chain |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:3,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.09% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody Read more... |
Note | For research use only |
Application notes | Anti-Mouse IgG2b Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
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