Mouse IgG2b antibody (ATTO 550)

• Catalog Number: orb347461
• Category: Antibodies
• Description: Mouse IgG2b antibody (ATTO 550)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG2b heavy chain
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, FC: 1:500 - 1:2,500, IHC: >1:5,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Conjugation: ATTO-550
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-MOUSE IgG2b Antibody ATTO550 Conjugation
• Note: For research use only
• Application notes: Anti-Mouse IgG2b ATTO 550 Antibody has been tested by dot blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.