MOUSE IgG2a antibody (ATTO 655)

• Catalog Number: orb347478
• Category: Antibodies
• Description: MOUSE IgG2a antibody (ATTO 655)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG2a heavy chain
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, FC: 1:500 - 1:2,500, IF: >1:5,000, WB: 1:4,000 - 1:20,000
• Form/Appearance: Lyophilized
• Purity: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Conjugation: ATTO-655
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-MOUSE IgG2a Antibody ATTO655 Conjugation
• Note: For research use only
• Application notes: Mouse IgG2a secondary antibody is available in a variety of formats. Anti-Mouse IgG2a ATTO 655 Antibody has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.