MOUSE IgG1 (Gamma 1 chain) antibody (ATTO 647N)

• Catalog Number: orb347471
• Category: Antibodies
• Description: MOUSE IgG1 (Gamma 1 chain) antibody (ATTO 647N)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG1 heavy chain
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, FC: 1:500 - 1:2,500, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Conjugation: ATTO-647N
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-mouse IgG1 antibody ATTO647N conjugation
• Note: For research use only
• Application notes: Anti-Mouse IgG1 ATTO 647N Antibody has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.