Mouse IgG1 antibody (FITC)

• Catalog Number: orb347372
• Category: Antibodies
• Description: Mouse IgG1 antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG heavy chain
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:200 - 1:500, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-Mouse IgG1 FITC conjugated secondary antibody prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-mouse IgG1 antibody fluorescein conjugat
• Note: For research use only
• Application notes: Mouse IgG1 FITC conjugated secondary antibody has been tested by western blot and is ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.