Mouse IgG1 antibody (Alkaline Phosphatase)

• Catalog Number: orb347389
• Category: Antibodies
• Description: Mouse IgG1 antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG1 heavy chain
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Goat anti-mouse IgG1 antibody alkaline phosphatase
• Note: For research use only
• Application notes: Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibody has been tested by ELISA and dot blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.