Mouse IgG (H&L) antibody (ATTO 532)

• Catalog Number: orb347457
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (ATTO 532)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Mouse IgG (H&L) Antibody ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
• Conjugation: ATTO-532
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti-Mouse IgG Antibody ATTO532 Conjugation,
• Note: For research use only
• Application notes: Anti-Mouse IgG (H&L) conjugated by ATTO 532 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of human umbilical vein endothelial cells using Mouse IgG (H&L) antibody (ATTO 532)