Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (ATTO 550)

• Catalog Number: orb347579
• Category: Antibodies
• Description: Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (ATTO 550)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
• Conjugation: ATTO-550
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain
• Note: For research use only
• Application notes: Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of rat kidney tissue using Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (ATTO 550)

Immunofluorescence analysis of human umbilical vein endothelial cells using Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (ATTO 550)