Mouse IgA Kappa isotype Control Antibody

SKU: orb343771

Description

Mouse IgA Kappa isotype Control Antibody

Images & Validation

• Tested Applications: ELISA, FC, SDS-PAGE
• Application Notes:
  Mouse IgA kappa isotype control has been tested in ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgA
• Purity: Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes size exclusion chromatography and antibody affinity chromatography. Analysis by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Concentration: 1.010 mg/mL
• Disclaimer: For research use only

Alternative Names

Mouse IgA istoype control, Mouse IgA subclass istoype control, Mouse IgA Kappa

IgG infusion in Aid−/− mice ameliorates the exacerbated HFDIO and promotes Treg cells in adipose tissue. Six-week-old male Aid + /+ and Aid−/− mice were fed with HFD for 13 weeks and received purified mouse IgG, IgA (p/n orb343771) or PBS by i.v. injection during the first two and last two weeks of the diet. (a) Depiction of the study design. (b) Proportional body weight change post-injection compared with starting body weight. (c–e) Proportion of total body fat assessed by Micro-CT (c) and weight of inguinal adipose tissue (d) and epididymal adipose tissue (e). (f, g) In vivo glucose (f) and insulin (g) tolerance responses from IgG-, IgA- or PBS-infused mice. Proportion of infiltrating Treg cells in the visceral fat, identified by flow cytometry, gated from live, single CD4+ T cells prior to gating on FoxP3 + cells (h). Data shown are pooled from two independent experiments. n = 6–8 per group. Data were assessed for significance using Student's t test (c–e, h) or two-way ANOVA (b, f, g). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. W, week.

SDS PAGE Results of Mouse IgA kappa Isotype Control. Lane 1: Mouse IgA kappa Isotype Control Reduced [1 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgA kappa Isotype Control Non-Reduced [1 µg]. 4-20% Gel, Coomassie Stained.