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Mouse IgA Kappa isotype Control Antibody
Catalog Number: orb343771
| Catalog Number | orb343771 |
|---|---|
| Category | Proteins |
| Description | Mouse IgA Kappa isotype Control Antibody |
| Isotype | IgA |
| Conjugation | Unconjugated |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Purity | Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain. |
| Tested applications | ELISA, FC, SDS-PAGE |
| Dilution range | FC: 1:1000-1:5000 |
| Application notes | Mouse IgA kappa isotype control has been tested in ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
| Source | Mouse |
| Biological Origin | Mouse |
| Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Alternative names | Mouse IgA istoype control, Mouse IgA subclass isto Read more... |
| Note | For research use only |
IgG infusion in Aid−/− mice ameliorates the exacerbated HFDIO and promotes Treg cells in adipose tissue. Six-week-old male Aid + /+ and Aid−/− mice were fed with HFD for 13 weeks and received purified mouse IgG, IgA (p/n orb343771) or PBS by i.v. injection during the first two and last two weeks of the diet. (a) Depiction of the study design. (b) Proportional body weight change post-injection compared with starting body weight. (c–e) Proportion of total body fat assessed by Micro-CT (c) and weight of inguinal adipose tissue (d) and epididymal adipose tissue (e). (f, g) In vivo glucose (f) and insulin (g) tolerance responses from IgG-, IgA- or PBS-infused mice. Proportion of infiltrating Treg cells in the visceral fat, identified by flow cytometry, gated from live, single CD4+ T cells prior to gating on FoxP3 + cells (h). Data shown are pooled from two independent experiments. n = 6–8 per group. Data were assessed for significance using Student's t test (c–e, h) or two-way ANOVA (b, f, g). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. W, week.
SDS PAGE Results of Mouse IgA kappa Isotype Control. Lane 1: Mouse IgA kappa Isotype Control Reduced [1 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgA kappa Isotype Control Non-Reduced [1 µg]. 4-20% Gel, Coomassie Stained.
