Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit

• Catalog Number: orb1950172
• Category: Assays and Kits
• Description: Mouse HSF1(Heat Shock Transcription Factor 1) ELISA Kit
• Reactivity: Mouse
• Range: 0.32-20 ng/mL
• UniProt ID: P38532
• Sensitivity: 0.138 ng/mL
• Alternative names: HSTF1; Heat shock factor protein 1
• Note: For research use only
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.