Mouse Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit

SKU: orb1173524

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Alpha A1(HSP90aA1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Alpha A1(HSP90aA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Alpha A1(HSP90aA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Alpha A1(HSP90aA1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP90aA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSP90aA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSP90aA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSP90aA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.64 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HSP86, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38

Quick Database Links

UniProt

P07901