Mouse Gamma-Aminobutyric Acid (gABA) ELISA Kit

SKU: orb782385

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gamma-Aminobutyric Acid(gABA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-Aminobutyric Acid(gABA).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse gABA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 9.66 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

4-Aminobutyric Acid ELISA Kit

References

Jiushuang Zhu 1, Zhuoting Zhong 1, Lijie Shi 1, Ling Huang 1, Chunqiao Lin 1, Yan He 1, Xiuwen Xia 1, Tiane Zhang 1, Weijun Ding 1, Youjun Yang Gut microbiota mediate early life stress-induced social dysfunction and anxiety-like behaviors by impairing amino acid transport at the gut Gut Microbes, (2024)

PubMed: 39259834

Applications

ELISA

Reactivity

Mouse