Mouse β-EP(Beta-Endorphin) ELISA Kit

• Catalog Number: orb1807724
• Category: Assays and Kits
• Description: Mouse β-EP(Beta-Endorphin) ELISA Kit
• Reactivity: Mouse
• Range: 15.63-1000pg/mL
• Target: β-EP
• Sensitivity: 9.38 pg/mL
• Alternative names: b-EP
• Note: For research use only
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. During the reaction, Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Expiration Date: Please enquire.