(Mouse) Dpf2 Antibody (N-term)

• Catalog Number: orb1787993
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 99-133 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC: 1:25, IHC: 1:25
• Conjugation: Unconjugated
• MW: 44230 Da
• Target: This mouse Dpf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 99-133 amino acids from the N-terminal region of mouse Dpf2.
• UniProt ID: Q61103
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Zinc finger protein ubi-d4, Apoptosis response zin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Dpf2 Antibody (N-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: mouse thymus lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Dpf2 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining (Mouse) Dpf2 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.