Mouse COX1(Cytochrome C Oxidase Subunit I) ELISA Kit

• Catalog Number: orb1088179
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit I(COX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit I(COX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit I(COX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit I(COX1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Range: 0.32-20 ng/mL
• UniProt ID: P00397
• Sensitivity: 0.122 ng/mL
• Protein Length: 3.5h
• Alternative names: MT-CO1, MTCO1, COI, COXI, Mitochondrially Encoded
• Note: For research use only
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Expiration Date: Please enquire.