Mouse CA19-9 ELISA Kit

• Catalog Number: orb1173595
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 19-9(CA19-9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-9(CA19-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 19-9(CA19-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 19-9(CA19-9) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 0.79-50 U/mL
• Sensitivity: 0.28 U/mL
• Protein Length: 3.5h
• Alternative names: sialylated Lewis(a)Antigen, Cancer Antigen 19-9
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Expiration Date: Please enquire.