Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit

• Catalog Number: orb1146806
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Range: 15.63-1000 pg/mL
• UniProt ID: P24288
• Sensitivity: 6.5 pg/mL
• Protein Length: 3.5h
• Alternative names: Branched-chain-amino-acid aminotransferase, cytoso
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.