Mouse Ascites Against Human IgG4 [Out of stock]

• Catalog Number: orb22214
• Category: Tools
• Description: Monoclonal mouse antibody to human IgG4, subclass specific. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: DOT, IEP
• Isotype: IgG1
• Concentration: IgG concentration is 1. mg/ml. No foreign proteins added.
• Source: Highly purified monoclonal IgG4 isolated from pooled human serum
• Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
• Buffer/Preservatives: Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
• Note: For research use only
• Application notes: The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Expiration Date: 12 months from date of receipt.