Mouse Anti-Human IgM antibody (FITC)

• Catalog Number: orb44862
• Category: Antibodies
• Description: Mouse Monoclonal to Human IgM.
• Clonality: Monoclonal
• Clone Number: CH2
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified human IgM.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: IgM
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IgM antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry multicolor staining pattern of human lymphocytes using anti-human CD19 (LT19) PE-Cy™7 antibody (surface staining, 4 µl reagent / 100 µl of peripheral whole blood) anti-human IgM (CH2) FITC antibody (intracellular staining, concentration in sample 3 µg/ml) after blocking of surface IgM using anti-human IgM (CH2) APC antibody (surface staining, concentration in sample 10 µg/ml).

Separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) FITC antibody (concentration in sample 1 µg/ml).